Yam meristem culture will grow in most basic plant tissue culture with minimal hormones such as MS Salts or Gamborg B5 heterotrophic media. As with any plant tissue culture media, growth is enhanced with the addition of plant growth regulations, and invariably that enhbacement is species and even cultivar dependent.
A basic MS Salts and vitamins meristem multiplication media for roughly bimonthly subculture is linked HERE in MS Word format that can be downloaded and costomized for the sotck solutions used in your specific lab.
A media for enhanced multiplication rates based on additional hormones is available ... LINK IT HERE
Todo: A spreadsheet containing alternative media formulations will be generated.
Inositol provides for buffering of phosphate uptake allowing for phytic acid formation (inositol hexaphosphate) thereby avoiding phoshpate toxicity.
Iron is provied with EDTA because Fe+3 is difficult to metabolize and keep in solution without a chelator of this nature. In fact the EDTA is provied in equimolar concentration with EDTA weather provided as as the sodium salt or the iron salt.
The ration of ammonium to nitrate facilitates pH contrl as ammonium metabolsim results in proton secretion, where nitrate metabolism involves the similutaneous uptake of a proton.